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Nicotine and related alkaloids are determined by gas chromatography with detectors such as Flame ionization detector (GC-FID), mass spectrometry (GC-MS), liquid chromatography tandem mass spectrometry (LC-MS/MS) etc.6a-b, 1a-b Tobacco specific nitrosoamines (TSNAs) are present in very low quantities and measured by liquid chromatography with quadruple mass detectors (LC-MS/MS).6c WHO TobLabNet official method SOP 05 can be used to analyse the benzo[a]pyrene in mainstream cigarette smoke.6dIn this method, the sample is extracted and analysed by GC-MS with electron ionization detection

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